Day 1 :
Keynote Forum
Sanjib Bhakta
Birkbeck, University of London and UCL, UK
Keynote: Repurposing common Non-Steroidal Anti- Inflammatory Drugs(NSAIDs) could potentially reverse intrinsic drug
Time : 10:00-11:00
Biography:
Sanjib Bhakta is a Reader (Associate Professor) in Molecular Microbiology and Biochemistry, Strategic Dean (International and Partnership) and Programme Director of MRes Global Infectious Diseases at the Institute of Structural and Molecular Biology, Birkbeck, University of London and UCL .His continued research interest in infectious bacterial diseases (funded by Wellcome Trust, Medical Research Council, UK and EU) is focussed on developing novel therapeutics as well as repurposing existing drugs to tackle antibiotic resistance and persistence in tuberculosis (TB), a global health emergency. He has published more than 100 original research articles for a number of internationally acclaimed journals including J. Exp. Med., JBC, Tuberculosis, Biochem. J., J. Antimicrob. Chemotherapy, FEBS J, Molecular Microbiology, British Medical Journal, PLOS, Journal of Medicinal Chemistry and Nature Sci Report.
Abstract:
The rise of antimicrobial resistance is leading to ever-more untreatable illness. Intracellularly surviving bacterial pathogens have endogenous machinery to evade host defenses as well as antibiotic treatment. Drug efflux and formation of biofilms are the two key fundamental mechanisms of intrinsic resistance which render many antibiotics ineffective against them. Mycobacterium tuberculosis has unique multi-drug transporter protein complexes that allow the pathogen to take up nutrients for survival, while allowing it to extrude deleterious ones so as the signaling molecules for quorum-sensing leading to biofilm formation. Our work has shown that the non-steroidal anti-inflammatory drugs (NSAIDs) have anti-bacterial action against Mycobacterium tuberculosis. The most potent NSAID so far, at sub-inhibitory concentrations, inhibited whole-cell efflux pumps activity at par with/better than potent efflux pump inhibitors such as verapamil and chlorpromazine. In addition, the NSAID inhibited mycobacterial biofilm formation significantly. Analysis of the extracellular polymeric substances of treated biofilm showed macromolecular alterations compared to the untreated controls. Furthermore, transcriptomic analysis revealed modulation of key metabolic pathways in NSAID-treated M. tuberculosis revealing novel endogenous targets of the drug. The over-the-counter immunomodulatory drug’s new antibiotic action has paved an alternative route for tackling antimicrobial resistance in TB.
- Pediatric, Bacterial and Infectious Diseases | Vector Borne Diseases | Virology and Infectious Diseaes | Pediatric Immunology and Medical Microbiology | Inflammatory and Neurological Infectious Diseases | Neonatal and Congenital Infections
Location: Conference Hall
Chair
Doshen Naidoo
Private Mother and Child Hospital, South Africa
Session Introduction
Martin R Evans
American Society for Microbiology, USA
Title: Proficiency testing/external quality assurance in the microbiology laboratory
Biography:
Dr Evans is a clinical laboratory director with a PhD in medical microbiology and immunology. He currently serves as a senior laboratory and informatics consultant to the Association of Public Health Laboratories (APHL) which is the parent organization of all state and local public health laboratories in the United States. During this time, he has been involved with APHL/CDC projects in Haiti, Kenya, Indonesia, Namibia, Uganda and Zimbabwe. As a microbiology and infectious disease consultant for the American Society for Microbiology, he has participated in CDC antimicrobial resistance surveillance and other programs in Ethiopia, Kenya and Bangladesh. Dr Evans serves on the New York State Board for Clinical Laboratory Technology and was the first chairman of the state board established in 2006. He is the recipient of APHL's Thomas E Maxson Education, Training and Workforce Development Award for significant contributions to Public Health Laboratory Practice. In 2013, Dr Evans concluded 6 years of public health laboratory service as an Associate Director at the New York City Public Health Laboratory.Key responsibilities included Director of Technical Affairs, Acting Manager of the Microbiology Section & LIMS Project Leader. Previously, Dr Evans spent 14 years in the private sector as a clinical laboratory director at Quest Diagnostics and SmithKline Beecham Clinical Laboratories. Prior to his work in the diagnostic laboratory, he was in academia including positions at Temple University and the University of Zimbabwe Faculty of Medicine. He is also a New York State licensed medical technologist.
Abstract:
Ana Rita de Toledo Piza
Limace Biotechnologia, Brazil
Title: New antiviral molecules from Phyllocaulis boraceiensis mucus
Biography:
Ana is the CEO / Scientific Director of Limace Biotecnologia, the first Brazilian company to develop pharmaceuticals and cosmetics from natural molluscan products. PhD in Biotechnology by the Institute of Biomedical Sciences of the University of São Paulo and master in Sciences by the Coordination of Disease Control (SES / SP). Post-doctoral study at the Butantan Institute, combining the study of molluscs with bioprospecting of antiviral compounds. Has experience in the field of macromolecule chemistry and bioprospecting active compounds from molluscs. He also works in the areas of zoology, microbiology, biochemistry, cell biology, virology and molecular phylogeny.
Abstract:
Terrestrial gastropods exude mucus by the body surface, when traveling, to protect its body from mechanical injury, desiccation or contact with harmful substances. Mucus of mollusks has been studied as a source of new natural compounds with diverse biological activities as its capability of inducing proliferation and remodelling tissue and their antiviral capacity. Fungus and viruses are related to a range of infectious diseases in humans and animals. Viruses cause worldwide outbreaks and pandemics in humans and animals every year with considerable morbidity and mortality. The molecular diversity of secondary metabolites extracted from mollusks is a good alternative for the discovery of novel bioactive compounds with unique structures and diverse biological activities. Phyllocaulis boraceiensis is a hermaphroditic slug that exudes mucus, in which was detected some molecules that exhibited potent antiviral activity against Measles, Influenza, Herpes, Rubella and Zika virus. In order to identify, isolate, purify and sequence molecules present in the mucus of the land slug P. boraceiensis with antiviral action "in vitro" were used fragmentation by chromatography and mass spectrometry in order to determine the active molecules and assay of biological activity, qPCR and Immunofluorescence labelling to determine the biological activity.
Sabine Vakou
Institut Pasteur de Cote d'Ivoire, Cote D'Ivoire
Title: Study of potentially pathogenicity of environmental mycobacteria non-ulcerans isolated in Cote D' Ivoire
Biography:
Abstract:
Kelvin Ngongolo
The Nelson Mandel African Institute of Science and Technology, Tanzania
Title: Influence of habitat and agricultural activities on the prevalence of trypanosome infection in cattle of Maasai Steppes, Tanzania
Biography:
Abstract:
Martin R Evans
American Society for Microbiology, USA
Title: Quality indicators in the microbiology laboratory: Establishing, monitoring and evaluation
Biography:
Abstract:
Olatoye Isaac Ajadi
University of KwaZulu-Natal, South Africa
Title: Investigation of nitric oxide, Proangiogenic and Antiangiogenic factors in serum from HIV infected pre-eclamptic South African women
Biography:
Abstract:
Fahad Alsufayan
National Guard Health Affairs, Saudi Arabia
Title: Infection control and prevention in NICU
Biography:
Abstract:
- Pediatric Healthcare and Nutrition | Pediatric Nursing and Surgery | Diagnosis, Prevention and Control of Infectious Diseases | Bacterial Pathogenesis and Microbial Genomics | Epidemiology and Emerging Tropical Diseases | HIV and Breast Feeding | Pharmacology and Nanotechnology in Pediatrics | Zoonotic Diseases and Influenza | Metabolic Disorders
Location: Conference Hall
Chair
Ana Rita de Toledo Piza
Limace Biotechnologia, Brazil
Session Introduction
Martin R Evans
Amerian Society for Microbiology, USA
Title: Establishing a training and competency program in the microbiology laboratory
Biography:
Abstract:
Aminu Mohammed
Ahmadu Bello University, Nigeria
Title: Ellagic acid modulates the oxidative stress profile in various organs of Trypanosoma congolense infected rats
Biography:
Abstract:
- Poster Presentation
Location: Conference Hall
Session Introduction
Iana SS Katz
Instituto Pasteur, Brazil
Title: Studies on the pathogenesis of street rabies virus strains associated with Insectivorous bats
Biography:
Abstract:
Biography:
Abstract:
Coulibaly Kalpy Julien
Institut Pasteur de Côte d'Ivoire, Côte d'Ivoire
Title: Microbiological and molecular characterisation of environmental Mycobacterium strains isolated from the buruli ulcer endemic and non-endemic zones in Cote D' Ivoire
Biography:
Abstract:
Ana Rita de Toledo Piza
Limace Biotechnologia, Brazil
Title: Zika virus infection control by Mollusk mucus
Biography:
Abstract:
Ana Rita de Toledo Piza
Limace Biotechnologia, Brazil
Title: Zika virus infection control by Mollusk mucus
Biography:
Abstract:
Mmabatho Peggy Mobo
Cape Peninsula University of Technology, South Africa